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Statistical differences between two groups for behavioral tests were determined with Student’s t test, and statistical differences among three groups or more were determined using a one-way analysis of variance (ANOVA), two-way ANOVA, and an ANOVA with repeated measures, followed by Bonferroni’s multiple comparison test

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The wound healing and regeneration effects operate through growth factor modulation and stem cell activation, with GHK-Cu shown promoting tissue repair, reducing inflammation, and supporting healthy cell turnover patterns